Fluoroskan ascent microplate fluorometer pdf

Detailed info and photos for this can be found on our website at fluoroskan ascent microplate reader fluorometer thrombinosc. Fluoroskan ascent fluorescence cf cell reading fluorescence. Under all conditions tested, extent of resazurin reduction was. Thermo scientific fluoroskan ascent microplate fluorometer.

The fluoroskan ascent fl fluoroskan ascent can carry out both top and bottom reading. Thermo scientific fluoroskan ascent fl from thermo fisher. Thermo scientific fluoroskan ascent or fluoroskan ascent fl, this reagent provides rapid and easy determination of dsdna, offering ultimate sensitivity and a wide dynamic range. Molecular bio products auto equip 1425442 fluoro filter for fluoroskan ascent microplate fluorometer, 544 nm excitation and emission. The fluoroskan ascent fl is equipped with both fluorometric and luminometric measurement technologies, providing a full coverage for fluorometric, glow and flash luminometric applications even in the same run. The compact fluoroskan ascent cf is simplified and standardized to focus specifically on cellbased requirements. Microplate fluorimetric assay of soil enzymes request pdf. Fluorometers fluorescence spectrophotometers a fluorescence spectrophotometer or fluorometer, fluorospectrometer, or fluorescence spectrometer measures the fluorescent light emitted from a sample at different wavelengths, after illumination with a xenon flash lamp.

Perform a variety of research applications with the compact, robust thermo scientific fluoroskan ascent microplate fluorometer. Molecular bio products auto equip 1425102 fluoro filter for fluoroskan ascent microplate fluorometer, 510 nm excitation and emission. The 374 fluoroskan ascent microplate fluorometer features excellent optical performance. It has a excitation range of 320nm700nm, and an emission range of 360nm670nm. Perform a variety of research applications with the compact, robust thermo scientific. Excellence for fluorometric and luminometric measurements. It features compatibility with 1 to 384 well plate formats and pcr plates, up to three onboard dispensers, an incubator and a shaker. Fluoroskan ascent microplate fluorometer the quantit picogreen dsdna quantitation reagent from invitrogen molecular probes is an ultrasensitive fluorescent stain used for the quantitation of doublestranded dna in a solution.

A fluorescencebased protocol for quantifying angiotensin. The fluorescence intensity was determined by a microplate fluorometer scientific fluoroskan ascent fl, thermo with 365 nm excitation and 450nm emission filters. Thermo scientific fluoroskan ascent microplate fluorometer is a compact and robust instrument with excellent optical performance for a variety of research applications. Thermo scientific fluoroskan ascent fl the thermo scientific fluoroskan ascent fl is a microplate fluorometer and luminometer for both luminometric and fluorometric research applications, such as protein and enzyme studies, molecular interactions, nucleic acid quantification, reporter gene, fluorometric kinase, and immuno and cell based assays. In fluorescence measurement, this prevents crosstalk and ensures accurate readings. Cell culture all melanoma cell lines in this study were established from metastasis of patients with stage iii or iv melanoma according to ajcc as previously described 17. Thermofisher scientific fluoroskan ascent fl fluorometer. Lc100 values, the concentrations of silver lethal to 100% of the cells, were determined from the doseresponse curves. Fluoroskan ascent microplate fluorometer automated fluorometer system with reagent dispenser and 384 well plates. Fluoroskan, fluoroskan fl, and luminoskan filterbased. Molecular bio products auto equip 1425442 fluoro filter for. The whole thermo scientific microplate reader family is compatible with skanit software.

Am, and livecell fluorescence was measured at 485 nm538 nm exem with the fluoroskan ascent microplate fluorometer labsystems. Thermo scientific accessories for fluoroskan ascent fl microplate fluorometer and luminometer products home. This microplate fluorometer is ideal for life science research applications such as fluorometric protein and enzyme studie. The fluoroskan ascent, a dedicated microplate fluorometer, offers high sensitivity for a wide variety of fluorometric applications. The onboard dispensers support simultaneous dispensing and reading, enabling the monitoring of fast kinetic measurements from the very start of the reaction. Thermo labsystems ascent family covers both fluorometry and luminometry with. These compact and robust instruments feature versatile plate formats, fast reading speeds, and excellent optical performance for a variety of fluorometric or luminometric research. In many molecular biology applications the detection and quantitation of small amounts of dsdna is very important.

Fluoroskan ascent microplate fluorometer pdf thermo scientific fluoroskan ascent microplate fluorometer. Fluoroskan ascent fl is a microplate fluorometer and luminometer with excellent optical performance for a variety of research applications, offering versatile plate formats, fast reading speed, up to three dispensers and top bottom reading of plates. L with 50 mm tris buffer ph 7 ionic strength adjusted to 100 mm with nacl with a fluorescein filter set ex. The flouroskan ascent fl is a microplate fluorometer and luminometer from thermoscientific. Fluoroskan ascent fl microplate fluorometer and luminometer. International journal of toxicology paradoxical role of 3. Microplate fluorometerluminometerfluoroskan ascent fl. The source of the excitation light and the detector of the emission light are both located on the same side of the microplate. Up to three reagent dispensers can be fitted onboard, making the reagent addition simple and highly accurate. Thus, the reader is ideal for applications such as reporter gene and cell adhesion assays, as well as cell proliferation and cytotoxicity assays. Fluoroskan ascent microplate fluorometer thermo fisher scientific with black, nonbinding 384 well plates greiner at a reaction volume of 60. High sensitivity luminometric and fluorometric assays in a. The thermo scientific fluoroskan ascent fl microplate reader is a. Our exclusive contracts with our clients yield a wide range of used microplate readers from a number of respected oems, including perkin elmer, tecan, molecular devices, biotek instruments, packard automation, labsystems and more.

Thermo labsystems 374 fluoroskan ascent microplate fluorometer. Fluoroskan ascent and fluoroskan ascent fl microplate. Electrophoresis, blotting and immunodetection plate based. The instrument is controlled with the flexible thermo scientific ascent software. Fluoroskan ascent microplate fluorometer from thermo. Thermo scientific fluoroskan ascent fl microplate fluorometer and luminometer is ideal for life science research applications. Equipnet is the worlds leading provider of used microplate readers and a number of other used equipment. Fluoroskan ascent microplate fluorometer fluoroskan ascent fl combined microplate fluorometer and luminometer reads 84well plates topbottom reading robotic compatible up to three dispensers incubation and shaking high sensitivity luminoskan ascent microplate luminometer reads 84well plates high. Microplate fluorometers for a variety of uses in the life science field, including dna, rna, and enzyme studies, feature enhanced reading speeds.

The fluoroskan ascent microplate fluorometer is specifically designed for life science research applications including molecular interactions, reporter gene, immuno and cell based assays, nucleic acid quantification. Spectrophotometers and spectrometers fluorescence spectrophotometers. Fluoroskan ascent microplate fluorometer luminoskan ascent microplate luminometer fluoroskan ascent fl combined microplate. Featuring excellent optical performance, the fluoroskan fl microplate fluorometer and luminometer is ideal for life science fluorometric and luminometric assays. Mar 10, 2019 fluoroskan ascent microplate fluorometer pdf thermo scientific fluoroskan ascent microplate fluorometer. Used thermo fluoroskan ascent 374 fluorometer for sale. Fluoroskan ascent and fluoroskan ascent fl microplate fluorometers, thermo scientific. Fluorescence measurements were carried out in a fluoroskan ascent microplate fluorometer using excitation and emission wavelengths of 355 and 405 nm, respectively. Thermo scientific accessories for fluoroskan ascent fl.

Fluoroskan fl microplate fluorometer and luminometer, 100240 v, 5060 hz with two dispensers 5200222 includes pc software and filter pairs. Fluoroskan fl microplate fluorometer and luminometer. Oct 04, 2019 fluoroskan ascent microplate fluorometer pdf. Used thermo scientific fluoroskan ascent microplate reader. Measurement with the fluoroskan ascent fl and fluoroskan ascent occurs in a narrow angle. The fluoroskan ascent microplate fluorometer is ideal for life science research applications, such as fluorometric. F luoroskan, fluoroskan fl, and l uminoskan microplate readers. The irrelevant mirnas celmir243 and athmir416 were used as negative controls. Leads require no additional cleaning or processing datashert used in soldered assembly. Fluoroskan ascent microplate fluorometer according to the manufacturers protocol. Appropriate cellfree controls were included to test for potential interaction between nac and pcn with resazurin. Ascent fl fluoroskan ascent can carry out both top and bottom reading.